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KMID : 0378019860290030123
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New Medical Journal
1986 Volume.29 No. 3 p.123 ~ p.131
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Effect of Heparin on pH, Pco₂ and Po₂ of Tonometered Whole Blood
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Abstract
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Heparin was widely used as an anticoagulant for blood sampling in the blood gas- an: lysis, Although it has been reported that the addition of excessive amounts of sodium. heparin . to blood samples could produce significant errors in pH and Pco2 of the arterial blood, the degree of changes of pH and Pco2 was variable according to authors. Also, the effect of. heparin on Po2 of the arterial blood was rarely reported.
This study was performed to observe the effect of the addition of heparin solution on .Po2 as well as pH and Pco2 of blood in the blood gas analysis, and to estim. te. the adequate amount of heparin solution to be used as the anticoagulant. The heparin solution - was so made to consisting 1000 units/ml of physiological saline. The 10 ml of veno ? blood -was drawn from the healthy college student with glass syringe. The syringe was applied to its wall and dead space with the heparin solution. The blood was equilibrated in the 1L 237 tonometer with the gas mixture of 5% CO2, 12% 02 and balance N2 for 30 minutes. To see the dilution effect, each sample of the same tonometered blood was dil ted by .adding 5, 10 and 20 volumes of the heparin solution per cent of the sampled blood. For contrast the other sample was diluted by adding each same volume of physiologica saline without heparin. And also, to see the effect of concentration of the heparin units during ,dilution by , adding the uniform 5 volumes of a heparin solution per cent of the sampled - blood, the concentration of the adding heparin solution was changed to 500, 1000, .2000 and 4000 units per milliliter respectively. The measurements were made of pH, Pco2 an, Po2 of. each diluted blood and the undiluted blood was measured as the control.
The results obtained are as follows:
When the blood was diluted by adding each 5, 10 and 20 volumes of the heparin olution per cent of the blood, the changes of pH was not significant comparing with tha of the control. Pco2 was decreased and Po2 was increased according to the dilution of t e blood with the heparin solution. The decrease of Pco2 was significant at the 10 and 20 plumes of the heparin solution, and the increase of Po2 was significant at the 20 volumes of the heparin solution.
Wheri the blood was diluted only with physiological saline, the changes of pH, Pco2 -nd Po2 of blood were similar values to that of the blood diluted with-the heparin solution.
When the concentration of the heparin units was varied as 500, 1000, 2000 and 4100 units, there were no significant changes of pH, Pco2 and Po2 of the blood.
In conclusion, it seems that the effect of heparin solution on Pco2 and Po2 of blooei is resulted from the dilution effect by the physiological saline within the heparin solut on rather than the effect of heparin itself. In the blood gas analysis it is recommended t at the heparin solution as an anticoagulant should be used less than 10 volumes per cent of the sampled blood regardless of the heparin unit.
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